Tissue Lipid Analysis with Enzymatic Reagents
نویسندگان
چکیده
منابع مشابه
Tissue lipid analysis with enzymatic reagents.
Featured Article: Carr TP, Andresen CJ, Rudel LL. Enzymatic determination of triglyceride, free cholesterol, and total cholesterol in tissue lipid extracts. Clin Biochem 1993;26:39 – 42. The need to measure serum or plasma cholesterol prompted the development of enzyme-based colorimetric assays during the 1970s. These assays eventually replaced the traditional chemical methods. The advantages o...
متن کاملReagents for (ir)reversible enzymatic acylations.
Commonly, hydrolase-catalysed reactions are used for kinetic resolutions of alcohols, amines and acids. Only a few applications in total syntheses have been described. Most of these examples are for the synthesis of amides and peptides. Here, the synthesis of all the different types of activated acids that can be applied in hydrolase-catalysed acylations is described. The application of these a...
متن کاملManual and centrifugal analyzer determination of cholesterol and triglycerides with single-vial enzymatic reagents.
We have assessed Calbiochem single-vial enzymatic reagent systems for the analysis for cholesterol and triglycerides, using manual and centrifugal analyzer techniques. Analysis for cholesterol by both techniques had acceptable performance by the short-term criteria of the Center for Disease Control (CDC) during this study carried out under optimal conditions of variance. Analysis for triglyceri...
متن کاملModeling Enzymatic Pathways in Giant Lipid Vesicles
Giant vesicles (GVs) are artificial chemical systems largely used as cell models [1-3], since they are micrometer-sized closed compartments bounded by a semi-permeable membrane, which in turn is composed by self-assembling amphiphiles. By opportunely engineering the lipid membrane and by entrapping cascade-reaction enzymes inside GVs, it comes possible to design micro-sized reactors where metab...
متن کاملAmyloglucosidase enzymatic reactivity inside lipid vesicles
Efficient functioning of enzymes inside liposomes would open new avenues for applications in biocatalysis and bioanalytical tools. In this study, the entrapment of amyloglucosidase (AMG) (EC 3.2.1.3) from Aspergillus niger into dipalmitoylphosphatidylcholine (DPPC) multilamellar vesicles (MLVs) and large unilamellar vesicles (LUVs) was investigated. Negative-stain, freeze-fracture, and cryo-tra...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2010
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2009.139360